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RBL cells : ウィキペディア英語版
RBL cells

RBL (Rat Basophilic Leukemia cells) 〔Bingham B.R., Monk P.N. and Helm B.A. (1994) Defective Protein Phosphorylation and Ca2+ Mobilization Linowa SecretingVariant of the Rat Basophilic Leukemia Cell Line. The Journal of Biological Chemistry: 269(30), pp. 19300-19306.〕〔http://www.hpacultures.org.uk/products/celllines/generalcell/detail.jsp?refId=86061001&collection=ecacc_gc〕 are cancerous basophil cells that are well suited for the study of allergy. They form a monolayer and are best cultured in Eagle's minimal essential medium with 10% Fetal bovine serum, 0.01 units/ml of penicillin and 0.1 mg/ml streptomycin incubated in 37°C + 5% CO2 + 90% relative humidity with a cell density of 5x105 cell per 15 ml.
==Uses==
RBL cells are suited for allergy studies specifically the IgE antibody and its FcεRI receptor because basophil cell are physiologically very similar to mast cells but are a much more stable cell line in tissue culture.

抄文引用元・出典: フリー百科事典『 ウィキペディア(Wikipedia)
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